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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Meio-Norte. |
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Data corrente: |
30/11/2015 |
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Data da última atualização: |
26/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENDES, R. F. M.; BRITTO, F. B.; FREIRE FILHO, F. R.; LIMA, P. S. da C. |
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Afiliação: |
R. F. M. Mendes, Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portella, Ininga, 64049-550, Teresina, PI; F. B. Britto, Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portella, Ininga, 64049-550, Teresina, PI; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; PAULO SARMANHO DA COSTA LIMA, CPAMN. |
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Título: |
Genetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
African Journal of Biotechnology, Nairobi, v. 14, n. 36, p. 2652-2661, Sep. 2015. |
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DOI: |
10.5897/AJB2015.14671 |
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Idioma: |
Inglês |
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Conteúdo: |
Cowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea populations (60 indigenous genotypes from 13 microregions of Rio Grande do Norte and 4 domesticated cultivars produced by Embrapa) using inter-simple sequence repeats (ISSR) markers. The set of 13 selected primers generated a total of 257 loci, 247 (96.11%) of which were polymorphic, with sizes ranging between 200 and 2000 bp. Genetic similarities between accessions were estimated from Jaccard coefficients and genetic relationships were determined from the dendrogram constructed using the unweighted pair group method with arithmetic average (UPGMA) technique. Bayesian statistics coupled with the Markov chain Monte Carlo technique was applied to determine population structure, while the genetic variability was established by analysis of molecular variance. UPGMA analysis allowed the separation of the genotypes into three groups, but no relationship between the genetic and geographical distances was observed. The fixation index was considered intermediary (FST = 0.0818), the average heterozygosity was low (HS = 0.39) and the coefficient of endogamy was high (f = 92.6%). The results show the presence of genetic diversity among the studied populations and revealed that such variability could be attributed mainly to intra-population variability (91.82%). MenosCowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea populations (60 indigenous genotypes from 13 microregions of Rio Grande do Norte and 4 domesticated cultivars produced by Embrapa) using inter-simple sequence repeats (ISSR) markers. The set of 13 selected primers generated a total of 257 loci, 247 (96.11%) of which were polymorphic, with sizes ranging between 200 and 2000 bp. Genetic similarities between accessions were estimated from Jaccard coefficients and genetic relationships were determined from the dendrogram constructed using the unweighted pair group method with arithmetic average (UPGMA) technique. Bayesian statistics coupled with the Markov chain Monte Carlo technique was applied to determine population structure, while the genetic variability was established by analysis of molecular variance. UPGMA analysis allowed the separation of the genotypes into three groups, but no relationship between the genetic and geographical distances was observed. The fixation index was considered intermediary (FST = 0.0818), the average heterozygosity was low (HS = 0.39) and the coefficient of endogamy was high (f =... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Drought stress; Estresse hídrico; Genetic diversity; Melhoramento de planta; Plant improvement. |
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Thesagro: |
Feijão de corda; Vigna Unguiculata. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134270/1/ArtigoSarmanhoAfricanJournalBiotechnology.pdf
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Marc: |
LEADER 02529naa a2200265 a 4500
001 2030738
005 2022-05-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.5897/AJB2015.14671$2DOI
100 1 $aMENDES, R. F. M.
245 $aGenetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers.
260 $c2015
520 $aCowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea populations (60 indigenous genotypes from 13 microregions of Rio Grande do Norte and 4 domesticated cultivars produced by Embrapa) using inter-simple sequence repeats (ISSR) markers. The set of 13 selected primers generated a total of 257 loci, 247 (96.11%) of which were polymorphic, with sizes ranging between 200 and 2000 bp. Genetic similarities between accessions were estimated from Jaccard coefficients and genetic relationships were determined from the dendrogram constructed using the unweighted pair group method with arithmetic average (UPGMA) technique. Bayesian statistics coupled with the Markov chain Monte Carlo technique was applied to determine population structure, while the genetic variability was established by analysis of molecular variance. UPGMA analysis allowed the separation of the genotypes into three groups, but no relationship between the genetic and geographical distances was observed. The fixation index was considered intermediary (FST = 0.0818), the average heterozygosity was low (HS = 0.39) and the coefficient of endogamy was high (f = 92.6%). The results show the presence of genetic diversity among the studied populations and revealed that such variability could be attributed mainly to intra-population variability (91.82%).
650 $aFeijão de corda
650 $aVigna Unguiculata
653 $aDiversidade genética
653 $aDrought stress
653 $aEstresse hídrico
653 $aGenetic diversity
653 $aMelhoramento de planta
653 $aPlant improvement
700 1 $aBRITTO, F. B.
700 1 $aFREIRE FILHO, F. R.
700 1 $aLIMA, P. S. da C.
773 $tAfrican Journal of Biotechnology, Nairobi$gv. 14, n. 36, p. 2652-2661, Sep. 2015.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Biblioteca |
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| Registros recuperados : 150 | |
| 11. |  | LIMA, P. S. da C.; BARRIGOSSI, J. A. F. Cultura do amendoim. In: SOUZA, V. A. B. de; COELHO, E. F. (org.). Tecnologias para produção em solos arenosos de tabuleiros costeiros do Meio-Norte. Teresina: Embrapa Meio-Norte, 2000. p. 89-107.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Meio-Norte. |
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| 13. | | DINIZ, F. M.; IYENGAR, A.; LIMA, P. S. da C.; MACLEAN, N.; BENTZEN, P. Application of a double-enrichment procedure for microsatellite isolation and the use tailed primers for high throughput genotyping. Genetics and Molecular Biology, São Paulo, v. 30, n. 2, p. 380-384, mar. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 20. | | GOMES, S. O.; MENDES, R. F. de M.; LIMA, P. S. da C. Repetibilidade de marcadores ISSR em acessos de pinhão-manso. In: CONGRESSO DA REDE BRASILEIRA DE TECNOLOGIA DE BIODIESEL, 5.; CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 8., 2012, Salvador. Biodiesel, inovação e desenvolvimento regional: anais, trabalhos científicos. Lavras: UFLA, 2012. p. 357-358| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 150 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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